![]() In order to make the technique more scientific rather than a mere interpretation by sight, what is called the Retention Value (Rf value for short) was applied in chromatography. Samples are usually of organic matter (not ionic salts) which dissolve in certain polar solvents (namely water) or non-polar (organic) solvents. Usually, one uses chromatography to find out the components of a sample which are seperated depending how much soluble these are in particular solvents and hence how far they travel along the chromatography paper. ![]() Chromatography - RF ValuesĪs described in the main chapter of this section, in paper chromatography there is what is known as the stationary phase which is the absorbent Chromatography paper and the mobile phase which is a liquid solvent (or mixture of solvents) used to carry the sample solutes under analysis along the paper.
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